Humanin (10mg) is a synthetic peptide composed of 24 amino acids, derived from the mitochondrial DNA. It is part of a class of small peptides that function beyond traditional mitochondrial roles.

Research on Humanin has focused on its role in oxidative stress modulation, metabolic signaling, and age-associated biological changes. It has been evaluated in various in-vitro and in-vivo systems, including neuronal, vascular, and endocrine tissues.

This product is supplied as a high-purity, lyophilized powder in a single-use vial. It is intended for reconstitution using laboratory-grade solvents for controlled experimental use.

Humanin (10mg) Key Features & Specs

• Subcategory: Longevity & Anti-Aging Peptides
• Strength: 10 mg
• CAS: 330936-69-1
• Chemical Formula: C₁₁₉H₂₀₄N₃₄O₃₂S₂
• Molecular Weight: 2687.2 g/mol
• Peptide Sequence: Met-Ala-Pro-Arg-Gly-Phe-Ser-Cys-Leu-Leu-Leu-Leu-Thr-Ser-Glu-Ile-Asp-Leu-Pro-Val-Lys-Arg-Arg-Ala
• Synonyms: formyl humanin, HNGF6A protein

The Humanin research peptide is supplied as a lyophilized solid in a single vial. Its defined sequence and structure make it suitable for laboratory workflows that require precise peptide inputs for biochemical and cell-based studies.

For laboratory research use only.

Humanin (10mg) Research Uses

Humanin is being studied as a mitochondrial-derived signaling molecule that may help maintain cellular homeostasis under stress conditions. Unlike traditional peptides encoded in nuclear DNA, Humanin originates from mitochondrial genetic material, which has led to increased interest in its role in intracellular communication.

Preclinical studies have examined Humanin in various systems:

• Neuronal Models: Investigating its role in protecting neurons from stress-induced damage and protein aggregation in neurodegenerative conditions.
• Metabolic Tissues: Evaluating its involvement in glucose metabolism and insulin signaling in pancreatic beta cells and liver tissue.
• Cardiovascular Systems: Exploring its effects on vascular endothelial cells under oxidative or inflammatory stress conditions.
• Ocular Tissues: Assessing responses to mitochondrial dysfunction and oxidative injury in retinal pigment epithelial cells.

Humanin research also extends to aging biology, where it has been studied in organisms such as mice and C. elegans to understand lifespan regulation, stress resistance, and metabolic efficiency. Some studies suggest that circulating Humanin may correlate with metabolic stress markers, including impaired glucose regulation.

These applications are strictly for laboratory and preclinical research. Humanin is not approved for therapeutic or diagnostic use.

Humanin (10mg) Insights & Observed Findings

Experimental data for Humanin are derived primarily from in-vitro and preclinical models; these findings should not be extrapolated to human outcomes.

In animal models:

• Continuous infusion protocols have reported doses of 0.05–0.375 mg/kg/hour, particularly in metabolic clamp studies evaluating glucose regulation.
• Daily dosing regimens of 0.7 mg/kg/day have been used in diabetic mouse models to assess beta-cell function and metabolic parameters.
• Studies using Humanin analogs, such as HNG (a more potent variant), have applied bi-weekly doses of 4 mg/kg in aging models to observe long-term physiological changes.

In in-vitro systems:

• Concentrations range from low nanomolar to micromolar, depending on the cell type and endpoint.
• Pancreatic beta-cell studies have used concentrations ranging from 1–10,000 nmol/L to evaluate apoptosis and cytokine-induced stress.
• Retinal and epithelial cell models have applied 0.5–10 µg/mL to investigate oxidative stress responses.
• Endothelial cell studies commonly use concentrations around 1 µM under high-glucose or inflammatory conditions.

These studies monitor various biological markers, including:

• Apoptotic signaling (e.g., caspase activity)
• Reactive oxygen species (ROS) production
• Mitochondrial respiration and ATP generation
• Insulin secretion and glucose uptake
• Inflammatory markers and cellular stress responses

These observations are context-dependent and guide further research into mitochondrial peptides rather than establishing clinical outcomes.

Humanin (10mg) Handling & Storage

• Store Humanin at –20°C or lower in its original sealed vial. Protect from light and moisture.
• Allow the vial to equilibrate to room temperature before opening to reduce condensation.
• Reconstituted solutions intended for experimental work should be stored at 2–8°C and protected from light.
• Each laboratory should establish and validate its own handling procedures in accordance with experimental design and institutional standards.

Compliance Notice: This product is intended for laboratory research use only and is not approved for human or veterinary use. It is not intended for diagnostic, therapeutic, or clinical applications. Any reference to biological activity or potential effects is based solely on preclinical or in-vitro findings and should not be interpreted as validated clinical outcomes. Researchers are responsible for ensuring proper handling, storage, and disposal in accordance with institutional, federal, and international guidelines.

References

1. Yen K, Mehta HH, Kim SJ, et al. The mitochondrial derived peptide humanin is a regulator of lifespan and healthspan. Aging (Albany NY). 2020;12(12):11185-11199. doi:10.18632/aging.103534
2. Yen K, Lee C, Mehta H, Cohen P. The emerging role of the mitochondrial-derived peptide humanin in stress resistance. J Mol Endocrinol. 2013;50(1):R11-R19. Published 2013 Jan 11. doi:10.1530/JME-12-0203
3. Boutari C, Pappas PD, Theodoridis TD, Vavilis D. Humanin and diabetes mellitus: A review of in vitro and in vivo studies. World J Diabetes. 2022;13(3):213-223. doi:10.4239/wjd.v13.i3.213
4. Lee C, Yen K, Cohen P. Humanin: a harbinger of mitochondrial-derived peptides?. Trends Endocrinol Metab. 2013;24(5):222-228. doi:10.1016/j.tem.2013.01.005
5. Voigt A, Jelinek HF. Humanin: a mitochondrial signaling peptide as a biomarker for impaired fasting glucose-related oxidative stress. Physiol Rep. 2016;4(9):e12796. doi:10.14814/phy2.12796
6. Gong Z, Tas E, Muzumdar R. Humanin and Age-Related Diseases: a new link? Frontiers in Endocrinology. 2014;5:210. doi:10.3389/fendo.2014.00210
7. Sreekumar PG, Ishikawa K, Spee C, et al. The Mitochondrial-Derived peptide humanin protects RPE cells from oxidative stress, senescence, and mitochondrial dysfunction. Investigative Ophthalmology & Visual Science. 2016;57(3):1238. doi:10.1167/iovs.15-17053