Epithalon (10 mg) is a synthetic tetrapeptide composed of the amino-acid sequence Ala-Glu-Asp-Gly (AEDG). The short Epithalon tetrapeptide was originally derived from research on pineal gland extracts known as epithalamin, which were studied for their role in aging-related-biological-process-related models and circadian signaling.

In laboratory investigations, Epithalon has been studied for its potential influence on cellular processes related to genomic stability, telomerase activity, and hormonal regulation. Current Epithalon research focuses on mechanisms of action in experimental systems, including telomere biology, gene expression, and circadian physiology.

Epithalon (10mg) Key Features & Specs

• Subcategory: Longevity & Anti-Aging Peptides
• Strength: 10 mg
• CAS: 307297-39-8
• Chemical Formula: C₁₄H₂₂N₄O₉
• Molecular Weight: 390.34 g/mol
• Peptide Sequence: Ala-Glu-Asp-Gly
• Synonyms: Epitalon, Epithalone, Epithalamin

For laboratory research use only.

Epithalon (10mg) Research Uses

Researchers have studied the Epithalon peptide across several biological systems related to aging-related processes, circadian biology, and cellular signaling. Because it originates from pineal peptide research, many experiments explore how the compound interacts with endocrine rhythms and genomic stability.

Areas commonly examined in Epithalon research include:

• Telomere and Cellular Aging Studies: Laboratory experiments investigating telomerase activity and telomere length in cultured human cells.
• Circadian Rhythm Research: Studies evaluating the relationship between peptide signaling and hormonal cycles, including melatonin production.
• Gene Expression Analysis: Investigations exploring how short regulatory peptides may influence transcription patterns in various tissues.
• Metabolic and Oxidative Stress Models: Experimental work assessing cellular-response-related readouts to age-related biochemical stress.

These studies aim to better understand how regulatory peptides may influence fundamental cellular processes in aging-related and physiological-regulatory-related models.

Epithalon (10mg) Insights & Observed Findings

Experimental findings provide insight into how the Epithalon tetrapeptide behaves in laboratory systems.

• In a study involving cultured human somatic cells, researchers observed that treatment with Epithalon was associated with activation of the telomerase enzyme and measurable telomere elongation in previously telomerase-negative cell lines. These findings suggested that the peptide may influence cellular-mechanism-related components involved in genomic-maintenance-related processes under experimental conditions.
• Additional investigations have examined endocrine and circadian effects. In animal studies involving aged rhesus monkeys, repeated administration of Epithalon was associated with changes in melatonin production and adjustments in circadian cortisol rhythms. Researchers interpreted these results as evidence that pineal-related peptides may influence hormonal-regulatory-related patterns.
• Further laboratory research has evaluated the peptide’s effects on gene transcription and cellular metabolism. In these studies, the Epithalon peptide was observed to alter expression patterns of genes related to cellular-structure-related organization, metabolic-regulation-related pathways, and stress-response-related markers.

While these observations are informative for mechanistic research, findings remain limited to experimental models and require further validation.

Epithalon (10mg) Handling & Storage

• Store Epithalon (10mg) in its lyophilized form at –20 °C or below in the original sealed vial. The vial should be protected from exposure to light and moisture before use.
• Allow the vial to reach room temperature prior to opening to help prevent condensation inside the container.
• Reconstitution should be performed using sterile, particle-free aqueous buffer while maintaining appropriate aseptic laboratory procedures.
• For use in the same experimental session or as specified by the investigator, reconstituted solutions are typically stored under refrigeration at 2–8 °C and protected from light.
• Follow institutional laboratory safety protocols and regulatory guidelines for disposal and handling.

Compliance Notice: This product is intended for laboratory research use only and is not approved for human or veterinary use. It is not intended for diagnostic, therapeutic, or clinical applications. Any reference to biological activity or potential effects is based solely on preclinical or in-vitro findings and should not be interpreted as validated clinical outcomes. Researchers are responsible for ensuring proper handling, storage, and disposal in accordance with institutional, federal, and international guidelines.

References

1. Araj SK, Brzezik J, Mądra-Gackowska K, Szeleszczuk Ł. Overview of Epitalon-Highly Bioactive Pineal Tetrapeptide with Promising Properties. Int J Mol Sci. 2025;26(6):2691. Published 2025 Mar 17. doi:10.3390/ijms26062691
2. Khavinson V, Diomede F, Mironova E, et al. AEDG Peptide (Epitalon) Stimulates Gene Expression and Protein Synthesis during Neurogenesis: Possible Epigenetic Mechanism. Molecules. 2020;25(3):609. Published 2020 Jan 30. doi:10.3390/molecules25030609
3. Caputi S, Trubiani O, Sinjari B, et al. Effect of short peptides on neuronal differentiation of stem cells. Int J Immunopathol Pharmacol. 2019;33:2058738419828613. doi:10.1177/2058738419828613
4. Khavinson VKh, Bondarev IE, Butyugov AA. Epithalon peptide induces telomerase activity and telomere elongation in human somatic cells. Bull Exp Biol Med. 2003;135(6):590-592. doi:10.1023/a:1025493705728
5. Anisimov VN, Khavinson VKh, Popovich IG, et al. Effect of Epitalon on biomarkers of aging, life span and spontaneous tumor incidence in female Swiss-derived SHR mice. Biogerontology. 2003;4(4):193-202. doi:10.1023/a:1025114230714